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Identification of a soil microorganism strain X-8, producer of beta-lactamase inhibitor, based on its morphological, physiological, biochemical and chemotaxonomical characterist ics was performed. The strain X-8 was identified as Pseudomonas sp. The beta-lactamase inhibitor produced by this strain was highly achieved in fermentation medium contained glucose 0.5%, urea 0.25%, K2HPO4.3H2O 0.5%, MgSO4.7H20 0.5%, FeSO4.7H20 0.01%, CuS04, ZnSO4, MnSO4 0.02%. The beta-lactamase inhibitor was not extracted by organic solvent such as n-butanol and ethyl acetate but remained in aqueous layer. The n-butanol extract showed antimicrobial activity against M. smegmatis. Ihe beta-lactamase inhibitor was stable at pH 7.0~8.0 and 4oC for 24h. The beta-lactamase inhibitor was bound on ion exchanger Diaion WA-30 and HP-20 and eluted with 2N-NH40H and acetone, respectively.
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